Tissue Engineering and Cellular Reprogramming

Overview

The core provides consultation, equipment, facilities and technical support for the generation and reprogramming of a wide range of viable embryonic and neural cell types in culture and in vivo and the analysis of integrative cellular, molecular and functional properties generated from these experimental paradigms. The core houses 2 six-foot and 1 four-foot Edgegard TC Laminar Flow Hoods, 2 Steril Gard III Biosafety Hoods, P2 facility, an EMS OTS-4000 Vibratome and 12 Forma Scientific Water Jacketed Tissue Culture Incubators equipped with separate O2, CO2, N2 and additional gaseous mixture and temperature controls to support these services.

Services

In vitro dissociated cell culture, slice and organotypic and whole embryo cultures
Embryonic stem (ES) cell experimental applications
In vivo real-time ultrasound-guided embryonic and adult stereotactic implantation surgeries using Visual Sonics Vevo 770 High Resolution In vivo Imaging
Human fetal cell experimentation
Gene expression quantification using ABI Prism 7000 Real Time PCR and ABI 6100 RNA prep station
mage analysis and data acquisition using Olympus BX51 and IX70 fluorescent microscopes associated with 2 Cooke Sensicam QE CCD digital cameras