Biohazard Flow Cytometry

Overview

The Biohazard Flow Cytometry Core provides high-speed cell sorting and multiparameter flow cytometric analysis for work involving agents posing a documented or potential biohazard. The laboratory is set up to enable high-speed sorting and analysis of unfixed human primary cells and cell lines under conditions that comply with safety standards recommended by the International Society for Analytical Cytology and approved by our Institutional Biosafety Committee. Resources are also available for sorting and analysis of various infectious agents, including many human pathogens such as HIV requiring up to Biosafety Level 2 containment.

Services

High-speed cell sorting using MoFlo-XDP or FACSAria
Multiparameter flow cytometric analysis using MoFlo-XDP, FACSAria, FACSCanto II and FACSCalibur
Laser scanning cytometry using Compucyte iCys
Magnetic antibody-bead-based sorting using Miltenyi Biotec SuperMACS
Consultation on biocontainment requirements for cell sorting experiments
Assistance with biohazard flow cytometry experimental design and data analysis
Training on operation of high-speed cell sorters and analyzers under biocontainment conditions