Flow Cytometry

Overview

The Flow Cytometry Core Facility provides a broad range of equipment and services for the analysis and isolation of cells and other similar-sized particles based on fluorescent labeling. Four high-speed cell sorters are available to enable optimized sorting of highly purified cells, subcellular organelles or bacteria. The facility also supports a variety of analytical flow cytometers capable of performing basic to advanced multiparameter fluorescence analysis of many types of cell suspensions as well as analysis of intracellular signaling. An experienced staff of technical experts provides instruction in the design, execution and analysis of flow cytometry-based studies.

Services

High-speed cell sorting using MoFlo and FACSAria cell sorters
Basic and advanced analytical flow cytometry using DXP10 Calibur, FACSCanto II and BD LSR-II analyzers
Laser scanning cytometry using Compucyte iCys
Magnetic antibody-bead-based sorting (Miltenyi Biotec SuperMACS)
Training in the use of all analytical instruments and FACSAria cell sorters
Resources and training for flow cytometry data analysis
Consultation on experimental design and protocol development
Seminars and training courses in basic, advanced and new emerging flow cytometry techniques