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Vladislav Verkhusha, Ph.D.

Vladislav Verkhusha, Ph.D.

Professor, Department of Anatomy & Structural Biology

Area of Research: Engineering of fluorescent proteins and biosensors for super-resolution microscopy and deep-tissue imaging. Designing of genetically encoded optogenetic tools for manipulation of biological processes with light in vivo.

Contact Information

718.430.8591vladislav.verkhusha@einsteinmed.org

Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Ullmann Building, Room 1217
Bronx, NY 10461

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Professional Interests

Non-invasive optical imaging, monitoring and manipulation of metabolic processes in living mammals is more feasible within the near-infrared (NIR) optical transparency window (650-900 nm) where hemoglobin and melanin absorbance significantly decreases, and water absorbance is still low. The most red-shifted fluorescent proteins (FPs) of the GFP-like family have excitation and emission spectra outside of the NIR region and suffer from low brightness and modest photostability. Natural bacterial phytochrome photoreceptors (BphPs) utilize an enzymatic product of heme, low-molecular weight biliverdin, as a chromophore.

BphPs provide many advantages over other natural chromophore containing proteins. Unlike the chromophores of non-bacterial phytochromes, biliverdin is ubiquitous in mammals. This makes BphP applications in mammalian cells, tissues and whole mammals as easy as conventional GFP-like FPs, without supplying chromophore through an external solution. BphPs exhibit NIR absorbance and fluorescence, which are red-shifted relative to that of any other phytochromes, and lie within the NIR optical window. This makes BphPs spectrally complementary to other existing optical probes and optogenetic tools based on the GFP, flavoprotein and rhodopsin-like protein families. Independent domain architecture and pronounced conformational changes upon biliverdin photoisomerization make BphPs attractive templates to design various photocontrollable genetically-encoded probes.

In our laboratory, we engineer new BphP-based FPs, biosensors and optogenetic tools. These include bright and spectrally resolvable permanently fluorescent NIR FPs, photoactivatable with non-phototoxic NIR light FPs, and reversibly photoswitchable FPs. We also focus on designing of NIR reporters for protein interactions and biosensors for intracellular ions and metabolites. Lastly, we engineer BphPs into optogenetic elements allowing to noninvasively regulate intracellular processes in vivo with NIR light.

We apply various directed protein evolution approaches based on rational structure-based design and random mutagenesis of template BphPs, high-throughput flow cytometry and multiwell plate spectroscopy. These conventional techniques allow screening for standard properties of genetically-encoded probes, such as excitation and emission wavelengths, brightness, photostability, pH stability and folding efficiency. We also develop new protein engineering and high-throughput approaches to specifically optimize BphP-based constructs. These include time-resolved fluorescence lifetime measurements, expression in bacterial periplasmic space, screening of mutant libraries in yeast and in mammalian cells using shuttle vectors and inducible somatic hypermutations.

The resulting NIR probes, biosensors and molecular tools are tested in mouse models and applied to various in vivo studies. These NIR constructs extend optical methods to multicolor deep-tissue in vivo imaging, cell and tissue labeling, photoactivation and tracking, and detection of enzymatic activities and protein interactions in cells, tissues and whole mammals. The engineered NIR optogenetic tools allow light-manipulations of cellular processes directly through the skin of living animals.

Selected Publications

Redchuk T.A., Karasev M.M., Donnelly S.K., Hülsemann M., Virtanen J., Moore H.M., Vartiainen M.K., Hodgson L. and Verkhusha V.V. Optogenetic regulation of endogenous proteins. Nature Communications 2020, 11: 605.

Matlashov M.E., Shcherbakova D.M., Alvelid J., Baloban M., Pennacchietti F., Shemetov A.A., Testa I. and Verkhusha V.V. A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales. Nature Communications 2020, 11: 239.

Leopold A.V., Chernov K.G., Shemetov A.A. and Verkhusha V.V. Neurotrophin receptor tyrosine kinases regulated with near-infrared light. Nature Communications 2019, 10: 1129.

Oliinyk O.S., Shemetov A.A., Pletnev S., Shcherbakova D.M. and Verkhusha V.V. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nature Communications 2019, 10: 279.

Shcherbakova D.M., Cammer N.C., Huisman T.M., Verkhusha V.V. and Hodgson L. Direct multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nature Chemical Biology 2018, 14: 591-600.

Li L., Shemetov A.A., Baloban M., Hu P., Zhu L., Shcherbakova D.M., Zhang R., Shi J., Yao J., Wang L.V. and Verkhusha V.V. Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo. Nature Communications 2018, 9: 2734.

Redchuk T.A., Kaberniuk A.A. and Verkhusha V.V. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nature Protocols 2018, 13: 1121-1136.

Redchuk T.A., Omelina E.S., Chernov K.G., and Verkhusha V.V. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nature Chem. Biol. 2017, 13: 633-639.

Yao J., Kaberniuk A.A., Li L., Shcherbakova D.M., Zhang R., Wang L., Li G., Verkhusha V.V. and Wang L.V. Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe. Nature Methods 2016, 13: 67-73.

Costantini L.M., Baloban M., Markwardt M.L., Rizzo M., Guo F., Verkhusha V.V. and Snapp E.L. A palette of fluorescent proteins optimized for diverse cellular environments. Nature Communications 2015, 6: 7670.

Shcherbakova D.M., Shemetov A.A., Kaberniuk A.A. and Verkhusha V.V. Natural photoreceptors as a source of fluorescent proteins, biosensors, and optogenetic tools. Annual Reviews of Biochemistry 2015, 84: 519-550.

In the News

More Media Coverage

Wednesday, December 06, 2017

BioTechniques features the research of Vladislav Verkhusha, Ph.D., in a story on advances in fluorescent dye and protein design for microscopy imaging. Dr. Verkhusha is professor of anatomy and structural biology.
Monday, August 01, 2016

Nature Methods profiles Vladislav Verkhusha, Ph.D., and his research, highlighting his creation of a new near-infrared optogenetic system.

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Vladislav Verkhusha, Ph.D.

Contact Information

Professional Interests

Selected Publications

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