Scientific Director: Ana Maria Cuervo, MD, PhD
Operations Manager: Simoni Tiano, simoni.tiano@einsteinmed.org
The Proteostasis of Aging Core provides state-of-the-art methodology for the study of changes in cellular protein homeostasis in aging and age-related disorders. The core performs highly specialized assays for the analysis of autophagic pathways and the ubiquitin proteasome system and provides advice on experimental design, techniques, and data interpretation in that area.
For information regarding consultation fees, experimental design and methods please contact ana-maria.cuervo@einsteinmed.org.
Fees (all fees are per assay)
| Type of service
|
NSC member rate
|
Others
|
| Protein turnover
|
| Complete Protein Turnover analysis
|
$600
|
$950
|
| Protein synthesis (4 samples with triplicates)
|
$200
|
$300
|
| Protein degradation (1 type sample 6 conditions with triplicates)
|
$500
|
$600
|
| Autophagy assays |
| Macroautophagy (MA)
|
| LC3 flux - immunoblot (8 conditions) |
$350 |
$550
|
| mCherry-GFP-LC3 High content microscopy/96 well plate |
$600
|
$800
|
| Mitophagy - immunofluorescence colocalization/8 well |
$300 |
$400 |
| Lipophagy - immunofluorescence colocalization/8 well |
$500 |
$750 |
| Em morphometric analysis of autophagic compartments*** |
$200 |
$300 |
| Chaperone-mediated autophagy (CMA)
|
| KFERQ-Dendra - High content microscopy/96 well plate
|
$600
|
$800
|
| Lysomal Function
|
| Lysosomal compartment – Lysotracker/ 8 well
|
$250
|
$250
|
| Lysosomal markers (panel) - Immunoblot
|
$300
|
$600
|
| Ubiquitin/Proteasome system assays
|
| Degron reporter – High content microscopy/ 96 –well plate
|
$650
|
$950
|
| Consultation
|
| Consultation on study design, methods, interpretation
|
$95/hr
|
$200/hr
|
| Training in proteostasis analysis procedures
|
$50/hr
|
$---
|
| Other Services
|
| Customized analysis of proteostasis
|
Negotiable
|
Negotiable
|
*** Includes morphometric analysis but excludes fees for sample processing and use of microscopes in the Analytical Imaging Facilities.