Health Span Core There are clear phenotypic changes that occur with aging, including shifts in energetics and metabolism, behavioral and functional deficits, and these phenotypic changes are accompanied by increased pathologic lesions and burden in tissues with advancing age. These effects can further be mediated by cell autonomous and cell non-autonomous mechanisms, which are interrogated via strategies, such as heterochronic parabiosis. To support demands for these services to the research community, the HSC was established as a critical resource within the E-NSC to perform integrative phenotyping of health and function across multiple domains in aging rodents by leveraging our long-standing expertise in conducting sophisticated in vivo metabolic studies, hormonal assays, and specialized surgeries, including parabiosis, as well as newly added services, including geropathology, behavior, and cardiovascular function. These studies are supported by an experienced and skilled team of staff scientists and technicians, and a distinguished group of expert consultants. The HSC also offers consultation, advice, and training to investigators interested in implementing these studies in their own lab, or to devise a customized study design for services in the Core. The HSC also makes an aging and parabiotic tissue repository available for dissemination to investigators upon request. Personnel Director: Derek M. Huffman, PhD Operations Manager: Dr. Kai Mao and Mr. Hongqian Liang Consultants: Dr. Nir Barzilai (glucose metabolism) Dr. Gary Schwartz (Whole-animal metabolic phenotyping) Dr. Rajat Singh (Cell metabolism assays) Dr. Maria Gulinello (Rodent behavior phenotyping) Dr. Richard Kitsis (Cardiovascular phenotyping) Dr. Amanda Beck (Geropathology) Services Assays In vivo clamp studies Specialized surgeries (catheters, ICV cannulas, parabiosis) Metabolic phenotyping Behavioral and functional phenotyping Geropathology Hormonal assays Reagents and Samples Aging and parabiotic tissue repository Prices BODY COMPOSITION AND DISSECTION Type of Service Member Rate Non-Member Rate qMR (rats or mice) $10 $30 Micro CT scan $50 $100 Complete dissection (weighing, fixation, freezing) $20 $35 SURGERIES Type of Service Member Rate Non-Member Rate Lipectomy $100 $150 Clamp surgery $100 $150 Inserting ICV or intra hypothalamic catheter $100 $150 Chronobiosis Surgery+Housing Y-Y, Y-O, or O-O each $200 $300 Chronobiosis Surgery+Housing (one set, i.e. Y-Y, Y-O, O-O) $500 $750 Chronobiosis limited tissue request (one set, i.e., Y-Y, Y-O, O-Y, O-O) $50 $100 Chronobiosis plasma/serum (one set, i.e., Y, Y-O, O-Y, O) ~50uL $100 $150 Chronobiosis complete tissue and plasma (one set, i.e., Y-Y, Y-O, O-Y, O-O) $600 $900 METABOLIC STUDIES Type of Service Member Rate Non-Member Rate Clamp assay: with radioisotope (mice) $200 $300 Clamp assay: with radioisotope/C14 (mice) $250 $350 Clamp assay: with radioisotope/C14 and ICV (mice) $300 $450 Clamp assay: with radioisotope (rats) $250 $400 Clamp assay: with radioisotope/C14 (rats) $300 $450 Clamp assay: with radioisotope/C14 and ICV (rats) $350 $500 Clamp data analysis $15 $25 Mouse Metabolic Cages (8 chambers per week) $75 $125 Rat Metabolic Cages (4 chambers per week) $100 $150 Hormonal assays (kit and reagent costs apply) inquire FUNCTIONAL ASSAYS Type of Service Member Rate Non-Member Rate FUNCTIONAL ASSAYS Balance Beam $60/hr $90/hr Gait $60/hr $90/hr Forced Exercise Test $60/hr $90/hr Grip Strength $60/hr $90/hr Rotarod $60/hr $90/hr Open Field $60/hr $90/hr Object Recognition $70/hr $120/hr Object Placement $70/hr $120/hr Y Maze/Zero Maze $60/hr $90/hr Frailty assessment $60/hr $90/hr Echocardiogram $80/hr $140/hr Unassisted Core Room Use $30/hr inquire GEROPATHOLOGY Type of Service Member Rate Non-Member Rate Geropathology analysis and report (4 tissues) $51 $100 Tissue processing, embedding, sectioning and Geropathology (4 tissues) $62.50 $115 CONSULTATION Type of Service Member Rate Non-Member Rate Consultation on study design, methods, interpretation $75/hr $120/hr Training in Core methodologies $60/hr $100/hr